ELISA User's Manual

List of Chapters

• List of Figures
• Introduction
  • Installation
  • Standard Curve Formation
  • Module Output
  • Dialog Windows
  • File Structures
• Module 1 – Process ELISA Reader Data
• Module 2 – Template Design
• Module 3 – Abstract Data
• Module 4 – Parameter Estimation
  • Standards File Selection
  • Coefficient Update
  • Number of Iterations
  • Pause after Iteration
  • Estimation Starting Values
  • Solution Nonconvergence
  • Analysis Strategies
  • Output Interpretation
  • References
• Module 5 – Plot Standard Curves
• Module 6 – Calculate Concentrations
  • Introduction
  • Opening Menu Dialog
  • Calculation Parameters
  • Results Window
• Module 7 – Edit Data Files
• Appendix A
• Appendix B

Figures

• Figure 1 — Uniform format for .DAT file required by program ELISA for Windows
• Figure 2 — File types used by program ELISA for Windows
• Figure 3 — ELISA for Windows opening menu screen
• Figure 4 — Dialog window for Module 1 – Process ELISA Reader Data
• Figure 5 — Raw data format – Dynatech MR5000 ELISA reader
• Figure 6 — Raw data format – Dynatech MR700 ELISA reader
• Figure 7 — Dialog window for Module 2 – Template Design
• Figure 8 — Display of sample template design
• Figure 9 — Dialog window for Module 3 – Abstract Data
• Figure 10 — Concluding message for Module 3 – Abstract Data
• Figure 11 — Dialog window for Module 4 – Parameter Estimation
• Figure 12 — Module 4 – ‘Pause after each iteration’ screen
• Figure 13 — Concluding message for Module 4 – Parameter Estimation
• Figure 14 — Sample output from Module 4 – Parameter Estimation
• Figure 15 — Dialog window for Module 5 – Plot Standard Curves
• Figure 16 — Typical plot of standards data overlaid with fitted standard curve
• Figure 17 — Diagram illustrating antibody concentration calculation for patient unknowns
• Figure 18 — Dialog window for Module 6 – Calculate Concentrations
• Figure 19 — Dialog window for optical density filter settings
• Figure 20 — Sample output from Module 6 – Calculate Concentrations
• Figure 21 — Logistic-log plot showing areas of antibody concentration unestimability
• Figure 22 — Sample summary (.SUM) file
• Figure 23 — Edit window from Module 7 – Edit Data Files
• Figure 24 — Display of sample template design